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Kulkarni, V. H.
- Anti-nociceptive Activity of Various Extracts of Dodonaea viscosa Jacq., Leaves
Abstract Views :499 |
PDF Views:387
Authors
S. D. Joshi
,
V. D. Kulkarni
,
V. H. Kulkarni
,
H. M. Vagdevi
,
V. P. Vaidya
,
V. P. Veerapur
,
A. M. Badiger
Source
Journal of Natural Remedies, Vol 6, No 2 (2006), Pagination: 135-140Abstract
Objective: The present study is aimed to evaluate the leaf extracts of Dodonaea viscosa for acclaimed antinociceptive activity on chemical and thermal induced pain. Materials and methods: Three experimental pain models viz. thermal stimulation by hot plate method, tail flick method using analgesiometer and writhing induced by glacial acetic acid were used for assessing antinociceptive activity of D. viscosa. Results: All the leaf extracts of D. viscosa showed antinociceptive activity in rats and mice. Conclusion: All tested extracts showed significant antinociceptive activity, among them ethyl acetate extract exhibited superior activity. Data suggests that the extracts of D. viscosa have an inhibitory activity on both peripheral and central pain mechanisms. The antinociceptive efficacy of the extracts may be attributed to the presence of flavonoids, sterols and saponins.Keywords
Dodonaea viscosa, Antinociceptive Activity, Ethyl Acetate ExtractFull Text
- Isolation, Characterization and Evaluation of Endophytic Fractions of Centella asiatica Linn. (Leaves) for Invitro Antioxidant Activity
Abstract Views :308 |
PDF Views:221
Authors
Affiliations
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
Source
Journal of Natural Remedies, Vol 20, No 1 (2020), Pagination: 29-41Abstract
In the present investigation, we have carried out the isolation of fungal endophytes from Centella asiatica Linn leaves followed by fermentation and extraction of fungal endophytes with non-polar solvents such as chloroform, ethyl acetate and n-butanol. Preliminary phytochemical investigation of endophytic crude fractions of leaves were also determined to detect the presence of primary and secondary metabolites followed by invitro free radical scavenging activity by reducing power, DPPH and hydroxyl radical assay. The chloroform fungal endophytic fractions were subjected to column chromatography by gradient elution technique for isolation of possible secondary metabolite. Reducing power of endophytic extracts of C. asiatica Leaf (CAL-1) (50-450 μg/ml) increased with increase in concentration. Reaction with DPPH radicals of CAL-1 showed good scavenging activity. The IC50 values for Ascorbic acid, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 30.33 μg/ml, 66.58 μg/ml, 79.33 μg/ml and 96.39 μg/ml respectively. In hydroxyl radical assay, The IC50 values for mannitol, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 121.06 μg/ml, 141.21 μg/ml, 181.80 μg/ml and 189.90 μg/ml respectively. The endophytic crude fractions of ethyl acetate exhibited potent antioxidant activity as compared to other fractions. Hence, ethyl acetate fungal endophytic fractions of Centella asiatica Linn leaves can be employed as a potential antioxidant in the prevention of oxidative stress caused by the free radicals.Keywords
Antioxidant, Centella asiatica, Column Chromatography, Endophyte.Full Text
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